発表論文
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発表論文

  1. Kawasaki T, Nishimura T, Tani N, Ramos C, Karaulanov E, Shinya M, Saito K, Taylor E, Ketting RF, Ishiguro K, Tanaka M, Siegfried KR, Sakai N. Meioc-Piwil1 complexes regulate rRNA transcription for differentiation of spermatogonial stem cells. eLife, 14:RP104295, 2025​​​.​​​​
  2. Kaneko, S, Miyoshi, K, Tomuro, K, Terauchi M, Tanaka R, Kondo S, Tani N, Ishiguro K, Toyoda A, Kamikouchi A, Noguchi H, Iwasaki S, Saito K. Mettl1-dependent m7G tRNA modification is essential for maintaining spermatogenesis and fertility in Drosophila melanogaster.  Nat. Commun., 15(1):8147, 2024.
  3. Yoshimura S#, Shimada R#, Kikuchi K, Kawagoe S, Abe H, Iisaka S, Fujimura S, Yasunaga K, Usuki S, Tani N, Ohba T, Kondoh E, Saio T, Araki K, Ishiguro K. Atypical heat shock transcription factor HSF5 is critical for male meiotic prophase under non-stress conditions. Nat. Commun., 15(1):3330, 2024. (#, Equally contributed) 研究紹介動画 YouTube
  4. Alavattam KG#, Esparza JM#, Hu M#, Shimada R#, Kohrs AR, Abe H, Munakata Y, Otsuka K, Yoshimura S, Kitamura Y, Yeh YH, Hu YC, Kim J, Andreassen PR, Ishiguro K*, Namekawa SH*. ATF7IP2/MCAF2 directs H3K9 methylation and meiotic gene regulation in the male germline. Genes Dev., 38(3-4):115-130, 2024. (#, Equally contributed; *, co-corresponding)
  5. Akiyama T, Ishiguro K, Chikazawa N, Ko SBH, Yukawa M, Ko MSH. ZSCAN4-binding motif – TGCACAC is conserved and enriched in CA/TG microsatellites in both mouse and human genomes.  DNA Res., 31(1):dsad029, 2024.
  6. Shimada R, Kato Y, Takeda N, Fujimura S, Yasunaga K, Usuki S, Niwa H, Araki K, Ishiguro K. STRA8–RB interaction is required for timely entry of meiosis in mouse female germ cells. Nat. Commun., 14(1):6443, 2023. 研究紹介動画 YouTube/研究紹介動画(英語) YouTube/研究紹介動画2(英語) YouTube
  7. Kuwayama N, Kujirai T, Kishi Y, Hirano R, Echigoya K, Watanabe S, Nakao M, Suzuki Y, Ishiguro K, Kurumizaka H, Gotoh Y. HMGA2 directly mediates chromatin condensation in association with neuronal fate regulation. Nat. Commun., 14(1):6420, 2023.
  8. Mwalilino L, Yamane M, Ishiguro K, Usuki S, Endoh M, Niwa H. The Role of Zfp352 in the regulation of transient expression of 2-cell specific genes in mouse embryonic stem cells. Genes Cells, 28(12):831-844, 2023.
  9. Frey T, Murakami T, Maki K, Kawaue T, Tani N, Sugai A, Nakazawa N, Ishiguro K, Adachi T, Kengaku M, Ohki K, Gotoh Y, Kishi Y. Age-associated reduction of nuclear shape dynamics in excitatory neurons of the visual cortex. Aging Cell, 22(9):e13925, 2023.
  10. Ishihara T, Fenelon J, Griffith O, Ishiguro K, Renfree M. Conserved H3K27me3-associated chromatin remodeling allows STRA8 but not MEIOSIN expression in mammalian germ cells. Reproduction, 165(5):507-520, 2023.
  11. Ito T, Ohta M, Osada A, Nishiyama A, Ishiguro K, Tamura T, Sekita Y, Kimura T. Switching defective/sucrose non-fermenting chromatin remodeling complex coordinates meiotic gene activation via promoter remodeling and Meiosin activation in female germline. Genes Cells, 28(1):15-28, 2022.
  12. Abe H, Yeh YH, Munakata Y, Ishiguro K, Andreassen PR, Namekawa SH. Active DNA damage response signaling initiates and maintains meiotic sex chromosome inactivation. Nat. Commun., 13(1):7212, 2022.
  13. Tani N, Tanno N, Ishiguro K. Tandem immuno-purification of affinity-tagged proteins from mouse testis extracts for MS analysis. STAR Protoc., 3(2):101452, 2022.
  14. Sano H, Nakamura A, Yamane M, Niwa H, Nishimura T, Araki K, Takemoto K, Ishiguro K, Aoki H, Kato Y, Kojima M. The polyol pathway is an evolutionarily conserved system for sensing glucose uptake. PLOS Biol., 20(6):e3001678, 2022.
  15. Oura S, Hino T, Satoh T, Noda T, Koyano T, Sotani A, Matsuyama M, Akira S, Ishiguro K, Ikawa M. Trim41 is required to regulate chromosome axis protein dynamics and meiosis in male mice. PLOS Genet., 18(6):e1010241, 2022.
  16. Hirano K, Nonami Y, Nakamura Y, Sato T, Sato T, Ishiguro K, Ogawa T, Yoshida S. Temperature sensitivity of DNA double-strand break repair underpins heat-induced meiotic failure in mouse spermatogenesis. Commun. Biol., 5(1):504, 2022.
  17. Saito Y, Santosa V, Ishiguro K, Kanemaki M. MCMBP promotes the assembly of the MCM2–7 hetero-hexamer to ensure robust DNA replication in human cells. eLife, 11:e77393, 2022.
  18. Kikuchi K, Sakamoto Y, Uezu A, Yamamoto H, Ishiguro K, Shimamura K, Saito T, Hisanaga S, Nakanishi H. Map7D2 and Map7D1 facilitate microtubule stabilization through distinct mechanisms to control cell motility and neurite outgrowth. Life Sci. Alliance, 5(8):e202201390, 2022.
  19. Tanno N, Takemoto K, Takada-Horisawa Y, Shimada R, Fujimura S, Tani N, Takeda N, Araki K, Ishiguro K. FBXO47 is essential for preventing the synaptonemal complex from premature disassembly in mouse male meiosis. iScience, 25(4):104008, 2022. 研究紹介動画YouTube
  20. Takeda I, Araki M, Ishiguro K, Ohga T, Takada K, Yamaguchi Y, Hashimoto K, Kai T, Yoshinobu K, Nakagata N, Imasaka M, Araki K. Gene trapping reveals a new transcriptionally active genome element: the chromosome-specific clustered trap region. Genes Cells, 26(11):874-890, 2021.
  21. Horisawa-Takada Y, Kodera C, Takemoto K, Sakashita A, Horisawa K, Maeda R, Shimada R, Usuki S, Fujimura S, Tani N, Matsuura K, Akiyama T, Suzuki A, Niwa H, Tachibana M, Ohba T, Katabuchi H, Namekawa S, Araki K, Ishiguro K.  Meiosis-specific ZFP541 repressor complex promotes developmental progression of meiotic prophase towards completion during mouse spermatogenesis. Nat. Commun., 12(1):3184, 2021. 研究紹介動画YouTube
  22. Takada Y, Yaman-Deveci R, Shirakawa T, Sharif J, Tomizawa S, Miura F, Ito T, Ono M, Nakajima K, Koseki Y, Shiotani F, Ishiguro K, Ohbo K, Koseki H. Maintenance DNA methylation in pre-meiotic germ cells regulates meiotic prophase by facilitating homologous chromosome pairing. Development, 148(10):dev194605, 2021. 
  23. Oura S, Koyano T, Kodera C, Takada Y, Matsuyama M, Ishiguro K, Ikawa M. KCTD19 and its associated protein ZFP541 are independently essential for meiosis in male mice. PLOS Genet., 17(5):e1009412, 2021.  
  24. Imai Y, SaitoK, Takemoto K, Velilla F, Kawasaki T, Ishiguro K, Sakai N. Sycp1 is not required for subtelomeric DNA double-strand breaks but is required for homologous alignment in zebrafish spermatocytes. Front. Cell Dev. Biol., 9:664377, 2021.
  25. Fujiwara Y, Horisawa-Takada Y, Inoue E, Tani　N, Shibuya H, Fujimura S, Kariyazono R, Sakata T, Ohta K, Araki K, Okada Y, Ishiguro K. Meiotic cohesins mediate initial loading of HORMAD1 to the chromosomes and coordinate SC formation during meiotic prophase. PLOS Genet., 16(9):e1009048, 2020.
  26. Tanno N, Kuninaka S, Fujimura S, Takemoto K, Okamura K, Takeda N, Araki K, Araki M, Saya H, Ishiguro K. Phosphorylation of the Anaphase Promoting Complex activator FZR1/CDH1 is required for Meiosis II entry in mouse male germ cell. Sci. Rep., 10(1):10094, 2020.
  27. Takemoto K, Tani　N, Takada Y, Fujimura S, Tanno N, Yamane M, Okamura K, Sugimoto M, Araki K, Ishiguro K. Meiosis-specific C19orf57/4930432K21Rik/BRME1 modulates localization of RAD51 and DMC1 to DSBs in mouse meiotic recombination. Cell Rep., 31(8):107686, 2020. 研究紹介動画YouTube
  28. Nakatake Y, Ko BHS, Sharov A, Wakabayashi S, Murakami M, Sakota M, Chikazawa N, Ookura C, Sato S, Ito N, Ishikawa-Hirayama M, Mak SS, Jakt LM, Ueno T, Hiratsuka K, Matsushita M, Goparaju SK, Akiyama T, Ishiguro K, Oda M, Gouda N, Umezawa A, Akutsu H, Nishimura K, Matoba R, Ohara O, Ko MSH. Generation and profiling of 2,135 human ESC lines for the systematic analyses of cell states perturbed by inducing single transcription factors. Cell Rep., 31(7):107655, 2020.
  29. Ishiguro K, Matsuura K, Tani N, Takeda N, Usuki S, Yamane M,  Sugimoto M,  Fujimura S,  Hosokawa M, Chuma S,  Ko S.H.M, Araki K, Niwa H. MEIOSIN directs the switch from mitosis to meiosis in mammalian germ cells. Dev. Cell, 52(4):429-445, 2020. Preview/研究紹介動画
  30. Takemoto K#, Imai Y#, Saito K, Kawasaki T, Carlton MP, Ishiguro K, Sakai N. Sycp2 is essential for synaptonemal complex assembly, early meiotic recombination and homologous pairing in zebrafish spermatocytes. PLoS Genet., 16(2):e1008640, 2020. (#, equally contributed)
  31. Hiratsuka K, Monkawa T, Akiyama T, Nakatake Y, Oda M, Goparaju SK, Kimura H, Chikazawa-Nohtomi N, Sato S, Ishiguro K, Yamaguchi S, Suzuki S, Morizane R, Ko BSH, Itoh H, Ko MSK. Induction of human pluripotent stem cells into kidney tissues by synthetic mRNAs encoding transcription factors. Sci. Rep., 9(1):913, 2019.
  32. Ida H, Akiyama T, Ishiguro K, Goparaju SK, Nakatake Y, Chikazawa NN, Sato S, Kimura H, Yokoyama Y, Nagino M, Ko MSH, Ko SBH. Establishment of a rapid and footprint-free protocol for differentiation of human embryonic stem cells into pancreatic endocrine cells with synthetic mRNAs encoding transcription factors. Stem Cell Res. Ther., 9(1):277, 2018.
  33. Ishiguro K, Nakatake Y, Chikazawa-Nohtomi N, Kimura H, Akiyama T, Oda M, Ko SBH, Ko MSH. Expression analysis of the endogenous Zscan4 locus and its coding proteins in mouse ES cells and preimplantation embryos. In Vitro Cell.Dev.Biol., 53(2):179-190, 2017.
  34. Ishiguro K, Monti M, Akiyama T, Kimura H, Chikazawa-Nohtomi N, Sakota M, Sato S, Redi CA, Ko SBH, Ko MSH. Zscan4 is expressed specifically during late meiotic prophase in both spermatogenesis and oogenesis. In Vitro Cell.Dev.Biol. 53(2):167-178, 2017.

 

 

<研究室立ち上げ以前の論文>

  1. (*: equally contributed)*Kim J, *Ishiguro K., Nambu A., Akiyoshi B., Yokobayashi S., Kagami A., Ishiguro T., Pendas A.M., Takeda N., Sakakibara Y., Kitajima T.S., Tanno Y., Sakuno T., Watanabe Y. : Meikin is a conserved regulator of meiosis-I-specific kinetochore function: Nature 517, 466-471 (2015)
  2. Kumar R., Ghyselinck N., Ishiguro K., Watanabe Y., Kouznetsova A., Höög C., Strong E., Schimenti J., Daniel K., Toth A., de Massy B.: MEI4: a central player in the regulation of meiotic DNA double strand break formation in mouse: J. Cell Science 128(9), 1800-1811 (2015)
  3. Ishiguro K., Kim J, Shibuya H, Hernández-Hernández A, Suzuki A, Fukagawa T, Shioi G, Kiyonari H, Li XC, Schimenti J, Höög C, Watanabe Y.: Meiosis-specific cohesin mediates homolog recognition in mouse spermatocytes. Genes&Development 28, 594-607 (2014)
  4. Shibuya H, Ishiguro K, Watanabe Y.: The TRF1-binding protein TERB1 promotes chromosome movement and telomere rigidity in meiosis. Nature Cell Biology 16:145-156 (2014)
  5. Belzil C., Asada N., Ishiguro K., Nakaya T., Parsons K., Pendolino V., Neumayer G., Mapelli M., Nakatani Y., Sanada K., Nguyen　MD. : p600 regulates spindle orientation in apical neural progenitors and contributes to neurogenesis in the developing neocortex. Biol.OPEN 8;3(6):475-485 (2014)
  6. (*: equally contributed) *Nakaya T., *Ishiguro K., Belzil C., Rietsch A.M., Yu Q., Mizuno S., Bronson R.T., Geng Y., Nguyen M.D., Akashi K., Sicinski P., Nakatani Y. : p600 Plays Essential Roles in Fetal Development., Plos One 8(6): e66269 (2013)
  7. Kim J., Ishiguro K., Kudo N., Watanabe Y. Meiosis specific cohesins in mouse embryonic oocytes. Methods in Molecular Biology 957: 47-57. (2012)
  8. Morimoto A, Shibuya H, Zhu X, Kim J, Ishiguro K, Han M, Watanabe Y. : A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis. J. Cell Biol.198:165-172 (2012)
  9. Ishiguro K., Kim J., Fujiyama-Nakamura S., Kato S., Watanabe Y. A new meiosis-specific cohesin complex implicated in the cohesin code for homologous pairing: EMBO report 12 : 267-275 (2011)
  10. Tanno Y., Kitajima T., Honda T., Ando Y., Ishiguro K., Watanabe Y.: Phosphorylation of mammalian Sgo2 by Aurora B recruits PP2A and MCAK to centromeres: Genes&Development 24 : 2169-2179 (2010)
  11. Kawashima SA, Yamagishi Y, Honda T, Ishiguro K, Watanabe Y. :Phosphorylation of H2A by Bub1 Prevents Chromosomal Instability Through Localizing Shugoshin. Science. 327 : 172-177 (2010)
  12. Shim SY., Wang J., Neumayer G., Tran H., Ishiguro K., Sanada K., Nakatani Y., Nguyen MD. : p600 is a Microtubule / Endoplasmic Reticulum-Associated Protein in CNS Neurons : J. Neuroscience 28 : 3604-3614 (2008)
  13. Kitajima TS, Sakuno T, Ishiguro K, Iemura S, Natsume T, Kawashima SA, Watanabe Y. : Shugoshin collaborates with protein phosphatase 2A to protect cohesin. Nature 441 : 46-52 (2006)
  14. Nakatani Y, Konishi H, Vassilev A, Kurooka H, Ishiguro K, Sawada J, Ikura T, Korsmeyer SJ, Qin J, Herlitz AM. : p600, a unique protein required for membrane morphogenesis and cell survival. Proc Natl Acad Sci USA.;102:15093-15098 (2005)
  15. (*: equally contributed) Ogawa H*, Ishiguro K.*, Gaubatz S., Livingston D., Nakatani Y. : A complex with chromatin modifiers that occupies E2F- and Myc-responsive genes in G0 cells, Science 296 : 1132-1136 (2002)
  16. Nagawa F., Kodama M., Nishihara T., Ishiguro K., Sakano H.: Footprint analysis of recombination signal sequences in the 12/23 synaptic complex of V(D)J recombination, Mol. Cell. Biol. 22 : 7217-7225 (2002)
  17. Yoshida T., Tsuboi A., Ishiguro K., Nagawa F., Sakano H.: The DNA bending protein, HMG1, is required for correct cleavage of 23bp-recombination signal sequences by RAG proteins in vitro, Int.Immunol. 12 : 101-109 : (2000)
  18. (*: equally contributed) Nagawa F.*, Ishiguro K.*, Tsuboi A.*, Yoshida T., Ishikawa A., Takemori T., Otsuka A.J., Sakano H. : Footprint analysis of the RAG protein recombination signal sequence complex for V(D)J type recombination, Mol. Cell. Biol. 18 : 655-663(1998)